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Worldwide first certified reference materials for GMO rapeseed powder mixtures

4 December, 2013

Genetically modified organisms (GMOs) need to be authorised before they are allowed to enter the European market. The availability of a reference material is one of the prerequisites for the authorisation of a GMO in Europe. DuPont Pioneer asked the European Commission's Joint Research Centre, Institute for Reference Materials and Measurements (JRC-IRMM) to develop a reference material for the 73496 GMO rapeseed event. This GMO rapeseed is designed to confer tolerance to the herbicide glyphosate.

Now the JRC-IRMM has released a set of five Certified Reference Materials (CRMs) ERM-BF434a-e, certified for their various mass fractions of the 73496 GMO rapeseed event. The challenge was to obtain sufficiently stable and homogenously mixed powders of 73496 GMO and non-GMO rapeseed. Therefore, a new grinding and mixing technique had to be developed in the course of this GMO CRM project. It allows JRC-IRMM to release the first rapeseed powder material certified for a GMO mass fraction world-wide.

The powder mixtures will enable GMO testing laboratories to carry out quality control and calibration of measurements for the implementation of the (EC) No 1829/2003 food and feed labelling threshold. In addition the availability of the CRMs ERM-BF434 is one of the prerequisites stipulated in the low level presence (LLP) legislation (EC) 619/2011 for feed.

The CRMs were prepared according to ISO Guides 34 by mixing gravimetrically dried GM and non-GM rapeseed powder. The resulting GM percentages were confirmed by real-time polymerase chain reaction (PCR) analysis after DNA extraction from the powders. The preparation and certification of the CRMs is described in detail in the certification report.

Latest update 4 December, 2013

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