Genetically modified organisms (GMOs) need to be authorised before they are allowed to enter the European market. The availability of a reference material is one of the prerequisites for the authorisation of a GMO in Europe. Dow AgroSciences (DAS) asked the European Commission's Joint Research Centre, Institute for Reference Materials and Measurements (EC JRC-IRMM) to develop a reference material for DAS-44406-6 soya. The DAS-44406-6 event is designed to confer tolerance to the glyphosate, glufosinate and 2,4-D herbicides.
The EC JRC-IRMM has released a set of five Certified Reference Materials (CRMs) ERM BF436a, b, c, d and e, certified for their DAS-44406-6 mass fractions. These reference materials will enable GMO testing laboratories to carry out measurements and to implement the (EC) No 1829/2003 food and feed labelling threshold.
The CRMs were prepared according to ISO Guides 34 by mixing gravimetrically dried GM and non-GM soya powder. The resulting GM percentages were confirmed by real-time polymerase chain reaction (PCR) analysis after DNA extraction from the powders. The preparation and certification of the CRMs is described in detail in the certification report.