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GMO certified reference materials for DAS-40278-9 maize : ERM-BF433

27 September, 2012

Legislation in the European Union regulates the placing on the market of food and feed consisting of, containing or produced from genetically modified organisms (GMOs). They are referred to as genetically modified (GM) food and feed and require labelling if they contain more than 0.9 % of GMOs. In general, this threshold demands on the one hand the development and validation of reliable methods for GMO quantification, and on the other hand the production of reference materials for calibration or quality control of these methods.

The series of GMO matrix reference materials for the maize event DAS-40278-9 (ERM-BF433) consists of four different mass fractions levels of DAS-40278-9 maize with a nominal value of 0, 5, 10 and 100 g/kg. The event is designed to confer tolerance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) and certain aryloxyphenoxypropionate (AOPP) herbicides (e.g. quizalofop) and received in accordance with Commission Regulation (EC) No 65/2004 the unique identifier code DAS-4Ø278-9.

ERM-BF433 was prepared according to ISO Guides 34 by mixing gravimetrically dried GM and non-GM maize powder. The resulting GM percentages were confirmed by real-time polymerase chain reaction (PCR) analysis after DNA extraction from the powders. The preparation and certification of ERM-BF433a, ERM-BF433b, ERM-BF433c and ERM-BF433d is described in detail in the certification report. 

Latest update 27 September, 2012

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