To prevent bovine spongiform encephalopathy (BSE) from spreading, the European Commission has taken several measures. The most important ones are the removing of specified risk material from the food chain, applying appropriate rendering conditions for the production of animal by-products and the ban of meat and bone meal (MBM) from mammals in compound feed for ruminants (Commission Regulation (EC) No 999/2001).
Due to a lack of appropriate animal specific tests, i.e. selective methods for detecting mammalian proteins in the presence of proteins from other animals, the ban on meat and bone meal was extended in 2001 to cover feed for almost all farmed animals (Commission Regulation (EC) No 1234/2003). The only official method in the EU for the detection of banned meat and bone meal in feed is microscopy (Commission Regulation (EC) No 152/2009), which does not yield precise information about the origin of the detected bones. Other methods are applied to the analysis of feed samples for the presence of banned processed animal proteins, such as methods based on: polymerase chain reaction (PCR), immunoassay and near infrared microscopy (NIRM).
Reports from IRMM:
C.von Holst, A.Boix, A.Marien, M.Prado. Factors influencing the accuracy of measurements with real-time PCR: The example of the determination of processed animal proteins. Food Control 24 (2012) p. 142-147
Fumiere O, Veys P, Boix Sanfeliu A, Von Holst C, Baeten V, Berben G. Methods of Detection, Species Identification and Quantification of Processed Animal Proteins in Feedingstuffs. Biotechnologie Agronomie Societe et Environnement, 13; 2009. p. 59-70.
Abbas O, Fernandez Pierna J, Boix Sanfeliu A, Von Holst C, Dardenne P, Baeten V. Key Parameters for the Development of a NIR Microscopic Method for the Quantification of Processed By-Products of Animal Origin in Compound Feedingstuffs. Analytical and Bioanalytical Chemistry 397 (5); 2010. p. 1965-1973.
Prado Rodriguez M, Fumiere O, Boix A, Marien A, Berben G, Von Holst C. Novel Approach for Interlaboratory Transfer of Real-time PCR Methods: Detecting Bovine Meat and Bone Meal in Feed. Analytical and Bioanalytical Chemistry 394 (5); 2009. p. 1423-1431.
Fumiere O, Veys P, Boix A, von Holst C, Baeten V, Berben G. Methods of detection, species identification and quantification of processed animal proteins in feedingstuffs. Biotechnol. Agron. Soc. Environ. 13(1); 2009, p. 57-68.
Von Holst C, Baeten V, Boix A, Slowikowski B, Pierna J, Tirendi S, Dardenne P. Transferability Study of a Near-Infrared Microscopic Method for the Detection of Banned Meat and Bone Meal in Feedingstuffs . Analytical and Bioanalytical Chemistry 392; 2008. p. 313-317.
Prado Rodriguez M, Berben G, Fumiere O, Van Duijn G, Mensinga-Kruize J, Reaney S, Boix A, Von Holst C. Detection of Ruminant Meat and Bone Meals in Animal Feed by Real-time Polymerase Chain Reaction (PCR): Results of an Interlaboratory Study. Journal of Agricultural and Food Chemistry 55; 2007. p. 7495-7501.
Van Raamsdonk L, Von Holst C, Baeten V, Berben G, Boix A, De Jong J. New Developments in the Detection and Identification of Processed Animal Proteins in Feeds. Animal Feed Science and Technology 133; 2007. p. 63-83.
See also FP6 projects Stratfeed and SAFEED-PAP.